PCT sensitive KRYPTOR > Measuring Principle
(for investigational use only)
KRYPTOR® uses TRACE (Time Resolved Amplified Cryptate Emission) technology, based on a non-radiative transfer of energy. This transfer takes place between two fluorescent tracers: europium cryptate (donor) and XL665 (acceptor). The signal measured during the formation of the antigen-antibody complex is accompanied by an amplification.
KRYPTOR® assays are homogeneous, without separation or washing. It is thus possible to obtain data without interrupting the immunological reaction. High-concentration samples are detected in the first few seconds of incubation, and may be diluted by the appropriate dilution factor, then re-assayed automatically.
The molecules of PCT present in the assay samples are sandwiched between the antibodies. The intensity of the signal is proportional to the amount of PCT. The shape of the standard curve is identical to that obtained by immunometric methods.
Fig.1 Measuring principle
