PCT
LIA > Luminometer Instrument
The Berthold LB 952 (T/16) luminometer
is our reference measuring instrument.
- The measuring instrument must be
able to accommodate 12 x 75 mm test tubes.
- Two injectors, either adjustable
or fixed, must be set to dispense an injection volume
of 300 ml and calibrated.
- The injection system must be corrosion-resistant,
because the reagents in the basic kit contain the
following ingredients:
BR1: 0.5% H2O2 in 0.1 M HNO
BR2: 0.25 M NaOH
- The instrument must have an uptake
mode to draw in new reagent solution and to rinse
the system with distilled water.
- It should be possible to set the
instrument so as to ensure a delay time of 1.20 seconds
between the first and second injections.
- The delay time between the second
injection and measurement should be 0.00 seconds.
The chemical reaction that leads to light emission
occurs in the first third of the second. In other
words, the second injection should occur directly
in the measurement position.
- Technical data on the photomultiplier
a. Chemical background should be < 100 RLU
b. Signal linearity of 100 to 700,000 RLU
c. Highest possible level of precision (RLU vs. VC)
